Extraction and sequencing of single nuclei from murine skeletal muscles

Extraction and sequencing of single nuclei from murine skeletal muscles

Blog Article

Summary: Single-nucleus RNA sequencing allows the profiling of gene expression in isolated nuclei.Here, we describe a step-by-step protocol optimized for adult mouse skeletal muscles.This Pens protocol provides two main advantages compared to the widely used single-cell protocol.First, it allows us to sequence the myonuclei of the multinucleated myofibers.

Second, it circumvents the cell-dissociation-induced transcriptional modifications.For complete details on the use and execution of this protocol, please refer to Dos Santos Mandrels et al.(2020) and Machado, Geara et al.(2021).